Research Question
- Can you verify Beer's Law through Colorimetry?
Abstract
· This experiment was conducted to learn how to make a stock solution for a serial dilution, and to understand the concepts of Beer's law through concentration and absorbance. The problem we tried to address was how to verify Beer's law. To solve this problem we used colorimetry.We diluted some Copper (II) Sulfate and created some serial dilution each with a different concentration of Copper (II) Sulfate and amounts of distilled water. He results we found were that the as the volume of distilled water increased the concentration of Copper (II) Sulfate decreased, and a decrease in absorbance. The overall conclusion I came up with was that the concentration of a solution is related to the amount of absorbance. As the concentration increases the more absorbance there are and vice versa.
Safety Concerns
Materials
· Spark System and Colorimeter
· Test Tube Holder
· 6 test tubes
· Distilled water (15 mL)
· Safety Goggles
· Copper (II) Sulfate (3.121g)
· 2 Pipets one for the water , and other for the stock solution
· a Graduated Cylinder
· Beaker
· Digital Scale
· 25mL Volumetric Flask with cork, for stock solution
· 6 Glass cuvettes
Procudure
Stock solution
1. Gather all the equipment, and put on the safety goggles.
2. You then must create and fill in the chart given to you like in Fig.1. You can’t fill in the read absorbance until you have created your dilution and started to measure absorbance.
3. You then have to create the stock solution, which starts out with you measuring the 3.121g of Copper (II) Sulfate with the digital Scale.
4. You then put the Copper (II) Sulfate into the volumetric flask and fill the flask to line, that represents 25mL, with distilled water.
5. Close flask with Cork and shake until all the Copper (II) Sulfate had dissolved in the water
Dilutions
1. Label the test tube 1-6
2. Using the chart you filled in you must now make the dilutions. The columns titled Volume of Stock Solution and Volume of Water is where you get the needed data to create the dilutions
3. In test tube 1 you are going to add 1 mL of Copper (II) Sulfate solution and 4 mL of distilled water using the pipet, to measure precisely you should use a different pipette for the water and stock
4. You must also use the graduated cylinders no measure exactly how much you need of the stock and water and also use different graduated cylinders to prevent contamination.
5. In the second test tube you add 2 mL of Copper (II) Sulfate solution and 3 mL of distilled water
6. In test tube 3 you add 3 mL of Copper (II) Sulfate solution and 2 mL of distilled water
7. In test tube 4 you add 4 mL of Copper (II) Sulfate solution and 1mL of distilled water
8. In test tube 5 you add 5 mL of Copper (II) Sulfate solution and no distilled water
9. In test tube 6 you add 5 mL of distilled water and no Copper (II) Sulfate Solution
Measuring Absorbance
1. You want to take the cord of the colorimeter and connect it to the spark system
2. Turn on the Spark system, and calibrate the Colorimeter by pressing the green button on the Colorimeter
3. Once the Spark system is calibrated then press the red absorbance and press show
4. This will lead you to a graph; you want to just see the percent of the red absorbances, so you press the arrow at the top of the screen.
5. Now that the Spark system is calibrated and set up you can start to measure the red absorbance of your serial solutions
6. You start by putting the solution of test tube1 in one of the Glass Cuvettes.
7. You place that Glass Cuvette into the colorimeter, close the lid, and start the recording of the red absorbance through the Spark system
8. You record your results in the chart given at the beginning of the experiment
9. You must continue steps 6-8 with test tubes 2-6
10. If you have a red absorbance level that is off just recalibrate the colorimeter using the solution of test tube 6.
11. Clean up you lab when you are all done by washing all of the glass and pipets and putting them away, also put away the spark system and colorimeter where they belong
12. Then using the data from the chart create a graph using the concentration of final solution and red absorbance
Evidence Collected
Figure 1
|
Test tube #
|
Concentration of stock solution
|
Volume of stock solution
|
Volume of water
|
Concentration of Final Solution
|
Volume of Final Solution
|
Red Absorbance
|
|
1
|
.5 mL
|
1 mL
|
4 mL
|
.1
|
5 mL
|
0.239
|
|
2
|
.5 mL
|
2 mL
|
3 mL
|
.2
|
5 mL
|
0.669
|
|
3
|
.5 mL
|
3 mL
|
2 mL
|
.3
|
5 mL
|
1.047
|
|
4
|
.5 mL
|
4 mL
|
1 mL
|
.4
|
5 mL
|
1.473
|
|
5
|
.5 mL
|
5 mL
|
0 mL
|
.5
|
5 mL
|
1.682
|
|
6
|
-----------------
|
0 mL
|
5 mL
|
0
|
5 mL
|
0
|
Figure 2
Claim
- With the data we collected we were able to verify Beer's law, which states that the higher concentration a solution has the higher the absorbance is. Figure 2 shows this data in a graph to show the relations.
Scientific Explanation
- I learned about the general concept of Beer's law. Beer's law pretty much states that the more concentrated a solution is the more light it absorbs. An example being if I had a highly concentrated solution of Iodine, the absorbance of the iodine would be high to since not a lot of light is able to pass through it. I can apply this information whenever I make a dilution. Knowing this I can somewhat predict the absorbance’s of a serial dilution. This law applies to life because doctors and nurses use it to test blood. they use it to make sure you have the right amount of nutrients in your blood. you could also use colorimetry to find someones blood alcohol concentration.